Saturday, November 21, 2009

Broadband Oscillator for proton decoupled 13C spectra

A very wide range of frequencies may be irradiated by using a broadband oscillator.
Proton decoupling is accomplished in the process of determining a 13C NMR spectrum by simultaneously irradiating all of the proton in the molecule with a broad spectrum of frequencies in the proper range.

Most modern NMR spectrometer provide a second tunable radio frequency generator, the decoupler for this purpose.

Irradiation causes the protons to become saturated and they undergo rapid upward and downward transitions, among all their spin states. These rapid transition decompose any spin-spin interaction between the hydrogen and 13C nuclei being observed. In effect, all spin interactions are averaged to zero by the rapid changes. The carbon nucleus senses only one average spin state for the attached hydrogen rather than two or more distinct spin states.

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