Fluoresence is caused by absorption of radiant energy and re-emission of some of this energy in the form of visible or UV light.
The light emitted always has higher wavelength than the incident light. In fluoresence the absorption and emission takes place with in a short time of the order of 10-12 s to 10-5 s. In phosphoresence the time delay is > 10-4 s to a few seconds. Both fluorescence and phosphorescence are put together as photo luminescence.
Intensity of fluorescent radiation is proportional to the concentration of the substance.
Applications of Fluorimetry:
Fluorimetry is used when there is no colorimetric method available, In inorganic chemistry the most frequent applications are for the determination of metal ions as fluorescent organic complexes. Uranium compounds fluoresce as such. Oxime (8-hydroxyl quinoline) complexes of Al, Zn, Mg fluoresce.
Other applications to determine quantitatively quinines, vitamins (B1 & B2).
The light emitted always has higher wavelength than the incident light. In fluoresence the absorption and emission takes place with in a short time of the order of 10-12 s to 10-5 s. In phosphoresence the time delay is > 10-4 s to a few seconds. Both fluorescence and phosphorescence are put together as photo luminescence.
Intensity of fluorescent radiation is proportional to the concentration of the substance.
Applications of Fluorimetry:
Fluorimetry is used when there is no colorimetric method available, In inorganic chemistry the most frequent applications are for the determination of metal ions as fluorescent organic complexes. Uranium compounds fluoresce as such. Oxime (8-hydroxyl quinoline) complexes of Al, Zn, Mg fluoresce.
Other applications to determine quantitatively quinines, vitamins (B1 & B2).
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